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Anti-CD31 Antibody (A88142)

Western Blot - Anti-CD31 Antibody (A88142) - Antibodies.com Western Blot - Anti-CD31 Antibody (A88142) - Antibodies.com Immunohistochemistry - Anti-CD31 Antibody (A88142) - Antibodies.com Immunohistochemistry - Anti-CD31 Antibody (A88142) - Antibodies.com Immunofluorescence - Anti-CD31 Antibody (A88142) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD31 for WB, IHC and ICC/IF.

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5-8 Business Days

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Highlighted products for Anti-CD31 Antibody

Flow Cytometry - Anti-CD31 Antibody [MEM-05] (A86185) - Antibodies.com
A86230
Anti-CD31 Antibody [MEM-05]
Mouse monoclonal [MEM-05] antibody to CD31 for Flow Cytometry, WB, IHC-P and ICC.
Flow Cytometry - Anti-CD31 Antibody [MEM-05] (A86185) - Antibodies.com
A86230
Anti-CD31 Antibody [MEM-05]
Mouse monoclonal [MEM-05] antibody to CD31 for Flow Cytometry, WB, IHC-P and ICC.
Name
Anti-CD31 Antibody
Description
Rabbit polyclonal antibody to CD31.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM (NP_000433.4).
Sequence
AKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
140 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD31 Antibody (A88142) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD31 Antibody (A88142)
Western blot analysis of extracts of THP-1 cells, using Anti-CD31 Antibody (A88142) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-CD31 Antibody (A88142) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-CD31 Antibody (A88142)
Western blot analysis of extracts of Mouse heart, using Anti-CD31 Antibody (A88142) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-CD31 Antibody (A88142) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD31 Antibody (A88142)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CD31 Antibody (A88142) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD31 Antibody (A88142) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD31 Antibody (A88142)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD31 Antibody (A88142) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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