This antibody reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells, dendritic cells, 25% of circulating T cells, and 15% of circulating B cells.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

HPB human acute lymphoid leukemia cell line.

Host

Mouse

Clonality

Monoclonal

Clone ID

MEM-260

Isotype

IgG1

Conjugate

APC

Excitation: 645nm, Emission: 660nm

Purification

This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Predicted MW

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

CD300 antigen, CD300 antigen-like family member A, CD300a antigen, CD300a molecule, CLM-8, CLM8_HUMAN, CMRF 35 H9, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, CMRF35H, CMRF35H leukocyte immunoglobulin like receptor, CMRF35H9, IgSF12, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, IRC1, IRC1/IRC2, IRC2, IRp60, Leukocyte membrane antigen, Mast cell derived paired immunoglobulin like receptor 1, NK inhibitory receptor, Polymeric immunoglobulin receptor 4

Isotype Controls