Anti-CD3 zeta (phospho Y153) Antibody [EM-17] (A86524)

Mouse monoclonal [EM-17] antibody to CD3 zeta (phospho Y153) for Flow Cytometry and WB.

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD3 zeta (phospho Y153) Antibody [EM-17]
Description
Mouse monoclonal [EM-17] antibody to CD3 zeta (phospho Y153).
Specificity
This antibody recognizes phosphorylated intracellular tyrosine 153 of CD3 zeta chain, which is a component of TCR/CD3 complex expressed on T cells.
Applications
Flow Cytometry, WB
Dilutions
WB: 2 - 5 µg/ml; Positive Control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, Non-reducing conditions recommended, Flow Cytometry: Intracellular staining: 1-4 µg/ml; Positive Control: Jurkat cells treated with pervanadate.
Reactivity
Human, Mouse
Immunogen
A phospho-specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH.
Host
Mouse
Clonality
Monoclonal
Clone ID
EM-17
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
4930549J05Rik, A430104F18Rik, AW552088, CD247, CD247 antigen, Cd247 molecule, Cd3, CD3 antigen, zeta polypeptide, isoform CRA_b, CD3 antigen, zeta subunit, CD3-eta, CD3H, CD3Q, Cd3z, CD3Z antigen zeta polypeptide (TiT3 complex), CD3zeta, CD3zeta chain, CD3Z_HUMAN, MGC140430, T cell receptor T3 zeta chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3z, TCR zeta chain, TCRk, TCRZ, TCRzeta
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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