Anti-CD3 zeta (phospho Y111) Antibody [EM-55] (A86526)

Name: Anti-CD3 zeta (phospho Y111) Antibody [EM-55]
Brand: Antibodies.com
SKU: A86526
Price: 195.0 USD
Availability: InStock

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$195

Mouse monoclonal [EM-55] antibody to CD3 zeta (phospho Y111) for Flow Cytometry and WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Specifications

Name

Anti-CD3 zeta (phospho Y111) Antibody [EM-55]

Description

Mouse monoclonal [EM-55] antibody to CD3 zeta (phospho Y111).

Specificity

This antibody recognizes phosphorylated intracellular tyrosine 111 of CD3 zeta chain, which is a component of TCR/CD3 complex expressed on T cells.

Applications

Flow Cytometry, WB

Dilutions

Flow Cytometry: 1-4 µg/ml; Intracellular staining.

Reactivity

Mouse, Human

Immunogen

BP1 (pTyr111/123) - KLH.

Host

Mouse

Clonality

Monoclonal

Clone ID

EM-55

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

4930549J05Rik, A430104F18Rik, AW552088, CD247, CD247 antigen, Cd247 molecule, Cd3, CD3 antigen, zeta polypeptide, isoform CRA_b, CD3 antigen, zeta subunit, CD3-eta, CD3H, CD3Q, Cd3z, CD3Z antigen zeta polypeptide (TiT3 complex), CD3zeta, CD3zeta chain, CD3Z_HUMAN, MGC140430, T cell receptor T3 zeta chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3z, TCR zeta chain, TCRk, TCRZ, TCRzeta

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

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Figure 1: Western Blot - Anti-CD3 zeta (phospho Y111) Antibody [EM-55] (A86526)

Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with Anti-CD3 zeta (Phospho-Tyr111) Antibody (A86526). 1: Wt mCD3zeta pervanadate non-stimulated. 2: Wt mCD3zeta pervanadate stimulated. 3: Mut. mCD3zeta Y/F2-6. 4: Mut. mCD3zeta Y/F1 and 3-6. 5: Mut. mCD3zeta Y/F1-2 and 4-6 (thus only pY111 remains native). 6: Mut. mCD3zeta Y/F1-3 and 5-6. 7: Mut. mCD3zeta Y/F1-4 and 6. 8: Mut. mCD3zeta Y/F1-5.

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