Anti-CD3 zeta Antibody [ARC0533] (A308361)

Name: Anti-CD3 zeta Antibody [ARC0533]
Brand: Antibodies.com
SKU: A308361
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0533] antibody to CD3 zeta for WB, IHC and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CD3 zeta Antibody [ARC0533]

Description

Rabbit monoclonal [ARC0533] antibody to CD3 zeta.

Applications

WB, IHC, Flow Cytometry

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, Flow Cytometry: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 65-164 of human CD3H (P20963).

Sequence

QQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0533

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

4930549J05Rik, A430104F18Rik, AW552088, CD247, CD247 antigen, Cd247 molecule, Cd3, CD3 antigen, zeta polypeptide, isoform CRA_b, CD3 antigen, zeta subunit, CD3-eta, CD3H, CD3Q, Cd3z, CD3Z antigen zeta polypeptide (TiT3 complex), CD3zeta, CD3zeta chain, CD3Z_HUMAN, MGC140430, T cell receptor T3 zeta chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3z, TCR zeta chain, TCRk, TCRZ, TCRzeta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CD3 zeta Antibody [ARC0533] (A308361)

Western blot analysis of extracts of Jurkat cells, using Anti-CD3 zeta Antibody [ARC0533] (A308361) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-CD3 zeta Antibody [ARC0533] (A308361)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD3 zeta Antibody [ARC0533] (A308361) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Flow Cytometry - Anti-CD3 zeta Antibody [ARC0533] (A308361)

Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD3 zeta Antibody [ARC0533] (A308361), (10 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line).

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Figure 4: Flow Cytometry - Anti-CD3 zeta Antibody [ARC0533] (A308361)

Flow cytometry analysis of Raji cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD3 zeta Antibody [ARC0533] (A308361), (2.5 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Raji cells was used as blank control (red line).

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