Anti-CD3 epsilon Antibody [CD3-12] (FITC) (A280085)

$215

Rat monoclonal [CD3-12] antibody to CD3 epsilon (FITC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD3 epsilon Antibody [CD3-12] (FITC)
Description
Rat monoclonal [CD3-12] antibody to CD3 epsilon (FITC).
Specificity
This antibody recognises human CD3e. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (e, ?, d, ?) that assemble and function as three pairs of dimers (e?, ed, ??). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).
Applications
Flow Cytometry
Dilutions
FC 1: 1:5 - 1:10
Reactivity
Human, Bovine, Dog, Horse, Rhesus Monkey, Pig, Chicken, Mouse, Duck, Koala, Harbour Porpoise, Alpaca, Cynomolgus monkey, Spotted Hyena, Sea Lion, Cat, Amazon Parrot, Mammals, Birds, Raccoon, Great horned owl (Bubo virginianus), Bullfrog, Xenopus, Amphibia, Rabbit, African green monkey
Immunogen
Synthetic peptide sequence ERPPPVPNPDYEPC from cytoplasmic epitope of CD3.
Sequence
ERPPPVPNPDYEPC
Host
Rat
Clonality
Monoclonal
Clone ID
CD3-12
Isotype
IgG1
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Rat anti Human CD3 antibody, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3e chain recognizes human CD3e. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (e, ?, d, ?) that assemble and function as three pairs of dimers (e?, ed, ??). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).
Synonyms
CD3e, CD3E antigen epsilon polypeptide, CD3e antigen epsilon polypeptide (TiT3 complex), CD3E antigen, epsilon subunit, CD3e molecule epsilon, CD3e molecule, epsilon (CD3 TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3E_HUMAN, IMD18, T cell antigen receptor complex epsilon subunit of T3, T cell surface antigen T3/Leu 4 epsilon chain, T cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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