Anti-CD3 Antibody [KT3] (FITC) (A280835)

Anti-CD3 Antibody [KT3] (FITC)

Rat monoclonal [KT3] antibody to CD3 (FITC).

This antibody recognises the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Mouse

CBAT6 thymocytes.

Rat

Monoclonal

KT3

IgG2a

FITC

Excitation: 490nm, Emission: 525nm

100 µg/ml

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.

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This product is for research use only. It is not intended for diagnostic or therapeutic use.