Anti-CD3 Antibody [CT-3] (A280150)

Anti-CD3 Antibody [CT-3]

Mouse monoclonal [CT-3] antibody to CD3.

This antibody recognises the chicken CD3 cell surface antigen expressed by T cells. The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.

Flow Cytometry, IHC-Fr, IP

Flow Cytometry: 1:50, Use 50µl of the suggested working dilution to label 10⁶ cells in 100µl

Chicken

Chicken thymocytes and Ig negative peripheral blood mononuclear cells.

Mouse

Monoclonal

CT-3

IgG1

Unconjugated

500 µg/ml

Liquid

Supplied in Borate Buffered Saline.

Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.

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