This antibody recognises mouse CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Applications
Flow Cytometry, Functional Assays
Dilutions
Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Mouse
Immunogen
Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Host
Mouse
Clonality
Monoclonal
Clone ID
E18
Isotype
IgG2b
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Synonyms
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
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