Mouse monoclonal [CD28.2] antibody to CD28 (APC-Cyanine 7).
Specificity
This antibody recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of MW 44 kDa) which is a critical costimulatory receptor of T cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA.
Host
Mouse
Clonality
Monoclonal
Clone ID
CD28.2
Isotype
IgG1
Light Chains
kappa
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD28 Antibody [CD28.2] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD28 Antibody [CD28.2] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
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