Anti-CD276 Antibody [DM53] (Biotin) - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM53] antibody to CD276 (Biotin).
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM53
Isotype
IgG
Conjugate
Biotin
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
1e5 of Expi 293 cell line were stained with 100µl of 1:1,000 diluted Anti-CD276 Antibody [DM53] (Biotin) - Azide free (A318642) (blue histogram) or isotype control (red histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.
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