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Anti-CD27 Antibody (A13763)

Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.com Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.com Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.com Immunohistochemistry - Anti-CD27 Antibody (A13763) - Antibodies.com Immunofluorescence - Anti-CD27 Antibody (A13763) - Antibodies.com Immunofluorescence - Anti-CD27 Antibody (A13763) - Antibodies.com Immunofluorescence - Anti-CD27 Antibody (A13763) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD27 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD27 Antibody
Description
Rabbit polyclonal antibody to CD27.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-191 of human CD27 (NP_001233.1).
Sequence
ATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIR
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD 27, CD27 antigen, CD27 molecule, CD27L receptor, CD27_HUMAN, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, Tumor necrosis factor receptor superfamily member 7
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD27 Antibody (A13763)
Western blot analysis of extracts of Raji cells, using Anti-CD27 Antibody (A13763) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-CD27 Antibody (A13763)
Western blot analysis of extracts of various cell lines, using Anti-CD27 Antibody (A13763) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-CD27 Antibody (A13763) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-CD27 Antibody (A13763)
Western blot analysis of extracts of Rat lung, using Anti-CD27 Antibody (A13763) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-CD27 Antibody (A13763) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD27 Antibody (A13763)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD27 Antibody (A13763) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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