This antibody reacts with an extracellular epitope of CD22, a 130 kDa type I transmembrane glycoprotein, of the immunoglobulin superfamily, expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes. It is lost on plasma cells, peripheral blood T lymphocytes, granulocytes, and monocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human cell line Reh.

Host

Mouse

Clonality

Monoclonal

Clone ID

IS7

Isotype

IgG1

Conjugate

APC

Excitation: 645nm, Emission: 660nm

Purification

This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Predicted MW

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

B cell receptor CD22 precursor, B lymphocyte cell adhesion molecule, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL CAM, BL-CAM, BLCAM, CD 22, CD22 antigen, CD22 molecule, CD22 protein, CD22_HUMAN, Lectin 2, Leu14, Lyb8, MGC130020, sialic acid binding Ig like lectin 2, Sialic acid binding immunoglobulin like lectin 2, Sialic acid-binding Ig-like lectin 2, SIGLEC 2, Siglec-2, SIGLEC2, T cell surface antigen Leu 14, T-cell surface antigen Leu-14

Isotype Controls