This antibody reacts with an extracellular epitope of CD21, a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells, and a subset of T lymphocytes. It is not expressed on immature B cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Porcine, Human, Canine, Bovine
Immunogen
IM9 human B-lymphoblastoid cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
LT21
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
145 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
