Recombinant Anti-CD21 Antibody [CR2/3124R] - BSA and Azide free (A251449)

Name: Anti-CD21 Antibody [CR2/3124R] - BSA and Azide free
Brand: Antibodies.com
SKU: A251449
Price: 525.0 USD
Availability: InStock

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$525

Recombinant rabbit monoclonal [CR2/3124R] antibody to CD21 for Flow Cytometry, IF, WB and IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Telephone

+1 (314) 370-6046

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Recombinant Antibody

Specifications

Name

Anti-CD21 Antibody [CR2/3124R] - BSA and Azide free

Description

Recombinant rabbit monoclonal [CR2/3124R] antibody to CD21.

Specificity

This antibody recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Applications

Flow Cytometry, IF, WB, IHC-P

Dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Reactivity

Human

Immunogen

Recombinant fragment, around amino acids 142-240, of human CD21 protein. The exact sequence is proprietary.

Host

Rabbit

Clonality

Monoclonal

Clone ID

CR2/3124R

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-CD21 Antibody [CR2/3124R] (A248267).

Synonyms

C3DR, CD 21, Complement C3d receptor, Complement C3d receptor 2, Complement component (3d/Epstein Barr virus) receptor 2, Complement receptor type 2, CR, Cr2, CR2_HUMAN, CVID7, EBV receptor, EBV-R, Epstein Barr virus receptor, Epstein-Barr virus receptor, EVBR, SLEB9

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunohistochemistry - Anti-CD21 Antibody [CR2/3124R] - BSA and Azide free (A251449)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil dendritic using Anti-CD21 Antibody [CR2/3124R].

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Figure 2: SDS-PAGE - Anti-CD21 Antibody [CR2/3124R] - BSA and Azide free (A251449)

SDS-PAGE analysis of Anti-CD21 Antibody [CR2/3124R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 3: Flow Cytometry - Anti-CD21 Antibody [CR2/3124R] - BSA and Azide free (A251449)

Flow cytometric analysis of PFA fixed MOLT4 cells using Anti-CD21 Antibody [CR2/3124R] followed by Goat Anti-Rabbit IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 4: Immunofluorescence - Anti-CD21 Antibody [CR2/3124R] - BSA and Azide free (A251449)

Immunofluorescent analysis of PFA fixed MOLT-4 cells stained with Anti-CD21 Antibody [CR2/3124R] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Nuclei are stained with RedDot.

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