This antibody recognises a glycoprotein antigen of ~105 kDa expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes. recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20. Identity of CD20 as the antigen recognized by Mouse anti Human B cells antibody, clone FMC7 was further confirmed by strong recognition of recombinant CD20 expressed in hematopoietic and non-haematopoietic cell lines and abolition of binding in CD20 extracellular domain mutations. The recognized epitope has also been shown to be cholesterol dependent.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.

Reactivity

Human

Immunogen

HRIK cells - human B-Lymphoblastoid line.

Host

Mouse

Clonality

Monoclonal

Clone ID

FMC7

Isotype

IgM

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Gel filtration of ascites.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in TRIS buffered glycine with 0.1% Sodium Azide and 0.2% BSA.

Storage

Shipped at ambient temperature. Store at 4°C. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Human B cells antibody, clone FMC7 recognizes a glycoprotein antigen of ~105 kDa expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes. Mouse anti Human B cells antibody, clone FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukemia and hairy cell leukemia.The expression pattern of the FMC7 antigen closely corresponds to that seen with CD22. Mouse anti Human B cells antibody, clone FMC7 recognizes a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20 (Serke et al. 2001). Identity of CD20 as the antigen recognized by Mouse anti Human B cells antibody, clone FMC7 was further confirmed by strong recognition of recombinant CD20 expressed in hematopoietic and non-haematopoietic cell lines and abolition of binding in CD20 extracellular domain mutations. The recognized epitope has also been shown to be cholesterol dependent (Polyak et al. 2003).

Synonyms

APY, ATOPY, B lymphocyte antigen CD20, B Lymphocyte Cell Surface Antigen B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, B1, Bp 35, Bp35, CD 20, CD20 antigen, CD20 receptor, CD20_HUMAN, CVID5, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU 16, LEU16, leukocyte surface antigen Leu 16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains A1, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1, MGC3969, MS4A1, MS4A2, S7

Isotype Controls