This antibody recognises the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that recognises human CD20 in its native oligomeric form.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or cells or 100µl whole blood

Reactivity

Human, Rhesus Monkey

Host

Mouse

Clonality

Monoclonal

Clone ID

2H7

Isotype

IgG2b

Conjugate

APC

Excitation: 645nm, Emission: 660nm

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with 1 ml of distilled water.

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Storage

Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).

Synonyms

APY, ATOPY, B lymphocyte antigen CD20, B Lymphocyte Cell Surface Antigen B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, B1, Bp 35, Bp35, CD 20, CD20 antigen, CD20 receptor, CD20_HUMAN, CVID5, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU 16, LEU16, leukocyte surface antigen Leu 16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains A1, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1, MGC3969, MS4A1, MS4A2, S7

Isotype Controls