Anti-CD18a Antibody [PNK-I] (PE) (A281579)

Anti-CD18a Antibody [PNK-I] (PE)

Mouse monoclonal [PNK-I] antibody to CD18a (PE).

This antibody recognises porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens. Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin ß2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity. CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules.CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Porcine, Camel

Porcine large granular lymphocytes.

Mouse

Monoclonal

PNK-I

IgG1

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Pig CD18a, clone PNK-I recognizes porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998). Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin ß2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity (Dato and Kim 1990).CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules.CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor (Chen et al. 2011).

This product is for research use only. It is not intended for diagnostic or therapeutic use.