Anti-CD18 Antibody [YFC118.3] - BSA and Azide free (A282036)

Anti-CD18 Antibody [YFC118.3] - BSA and Azide free

Rat monoclonal [YFC118.3] antibody to CD18.

This antibody demonstrates strong reactivity with leucocytes and is not reactive with platelets.

IHC-Fr, IF, Flow Cytometry, IP, Functional Assays

Flow Cytometry: 1:50 - 1:100, IHC-Fr 1: 1:1,000 - 1:5,000, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human, Dog, Guinea Pig

Human neutrophils.

Rat

Monoclonal

YFC118.3

IgG2b

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Human CD18 antibody, clone YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigens (code number N221) as recognizing the CD18 antigen. CD18 is an integral membrane glycoprotein of ~95 kDa, also known as the beta 2 chain, of the LFA-1 complex. CD18 links non-covalently to either CD11a, b or c molecules forming the heteromeric LFA-1 complex. CD18 acts as the receptor for ICAM-1 and is important for cell adhesion and cell-cell interactions.

Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta, Integrin beta-2, ITGB2, MFI7