This antibody recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen. CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Cross Reactivity

This antibody does not cross react with Dog.

Immunogen

Leukocytes from a patient suffering from a LGL Type leukemia.

Host

Mouse

Clonality

Monoclonal

Clone ID

MEM-48

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Predicted MW

90-95 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

95 subunit beta, Beta 2 (antigen CD18 (p95), CD 18, Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor), Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement component 3 receptor 3 and 4 subunit, Complement receptor C3 beta subunit, Complement receptor C3 subunit beta, Integrin beta 2, Integrin beta chain beta 2, Integrin beta-2, Integrin beta-2 (ITGB2), Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), ITB2_HUMAN, ITGB2, LAD, LCAMB, Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C, Leukocyte cell adhesion molecule CD18, LFA 1, LFA1, Lymphocyte function associated antigen 1, Lymphocyte function associated antigen 1 (LFA1), MAC 1, MAC1, Macrophage antigen 1 (mac1) beta, MF17, MFI7, OTTHUMP00000115278, OTTHUMP00000115279, OTTHUMP00000115280, OTTHUMP00000115281, OTTHUMP00000115282

Isotype Controls