Anti-CD163 Antibody [EDHu-1] (A280344)

Anti-CD163 Antibody [EDHu-1]

Mouse monoclonal [EDHu-1] antibody to CD163.

This antibody recognises the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone.Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163. Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.

Flow Cytometry, WB, ELISA, IHC-Fr, IHC-P, IF, Immunoassay

Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human, Rhesus Monkey, Sheep, Pig, Guinea Pig, Bovine, Cynomolgus monkey

Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax.

Mouse

Monoclonal

EDHu-1

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone.Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsen et al. 2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.

C163A_HUMAN, CD 163, CD163 antigen, CD163 molecule, Hemoglobin scavenger receptor, M130, M130 antigen precursor, Macrophage associated antigen, MM130, OTTHUMP00000238617, OTTHUMP00000238618, OTTHUMP00000238619, OTTHUMP00000238620, SCARI1, Scavenger receptor cysteine rich type 1 protein M130, sCD163, Soluble CD163