Anti-CD163 Antibody [2A10/11] (FITC) (A279159)

Anti-CD163 Antibody [2A10/11] (FITC)

Mouse monoclonal [2A10/11] antibody to CD163 (FITC).

This antibody recognises porcine CD163, a ~120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor. Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to 1x10⁶ cells in 100µl

Porcine

Porcine alveolar macrophages.

Mouse

Monoclonal

2A10/11

IgG1

Protein A affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a ~120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor (Sanchez et al. 1999). Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torres et al. 2003).

C163A_HUMAN, CD 163, CD163 antigen, CD163 molecule, Hemoglobin scavenger receptor, M130, M130 antigen precursor, Macrophage associated antigen, MM130, OTTHUMP00000238617, OTTHUMP00000238618, OTTHUMP00000238619, OTTHUMP00000238620, SCARI1, Scavenger receptor cysteine rich type 1 protein M130, sCD163, Soluble CD163

This product is for research use only. It is not intended for diagnostic or therapeutic use.