This antibody recognises the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a single C-type lectin domain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa. CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function. recognises a similar epitope to the DX1 monoclonal antibody.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reactivity

Human

Immunogen

Purified human NK cells cultured in IL-2.

Host

Mouse

Clonality

Monoclonal

Clone ID

B199.2

Isotype

IgG1

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with 1 ml of distilled water.

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Mouse anti Human CD161 antibody, clone B199.2 recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a single C-type lectin domain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa (Lanier et al. 1994). CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function (Aldemir et al. 2005).Mouse anti Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanier et al. 1994).

Synonyms

C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer Cell Lectin like Receptor Subfamily B Member 1, Killer cell lectin-like receptor subfamily B member 1, KLRB1, KLRB1_HUMAN, Natural killer cell surface protein P1A, NKR, NKR P1, NKR-P1A, NKRP1, NKRP1A

Isotype Controls