This antibody recognises porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens. Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells. Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reactivity

Porcine

Immunogen

Porcine Peripheral Blood Leucocytes.

Host

Mouse

Clonality

Monoclonal

Clone ID

Isotype

IgG1

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with 1 ml of distilled water.

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Storage

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).

Synonyms

CD 16, CD 16a, CD16a, CD16a antigen, CD16B, CD16b antigen, Fc fragment of IgG, Fc fragment of IgG low affinity IIIa receptor, Fc fragment of IgG low affinity IIIa receptor (CD16), Fc fragment of IgG receptor IIIa, Fc fragment of IgG, low affinity III, receptor (CD16), Fc fragment of IgG, low affinity III, receptor for (CD16), Fc fragment of IgG, low affinity IIIa, receptor (CD16), Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for, Fc fragment of IgG, low affinity IIIb, receptor (CD16b), Fc fragment of IgG, low affinity IIIb, receptor for (CD16), Fc gamma R3, Fc gamma receptor III 2 (CD 16), Fc gamma receptor III A, Fc gamma receptor IIIA, Fc gamma receptor IIIb (CD 16), Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIII beta, Fc gamma RIIIa, Fc gamma RIIIb, Fc of IgG, Fc-gamma receptor III2 (CD 16), Fc-gamma receptor III2 (CD16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG 3, FCG3, FCG3A_HUMAN, FCgammaRIIIA, FCGR 3, FCGR 3A, FCGR3, FCGR3A, FCGR3A protein, FCGRIII, FCGRIII-2, FcR 10, FcR-10, FcR10, FcRIII, FcRIIIa, IGFR 3, IGFR3, IgG Fc receptor III 1, IgG Fc receptor III 2, IgG Fc receptor III-2, IMD20, immunoglobulin G Fc receptor III, immunoglobulin G Fc receptor III-2, Low affinity IIIa receptor, Low affinity immunoglobulin gamma Fc region receptor III A, Low affinity immunoglobulin gamma Fc region receptor III-A, Low affinity immunoglobulin gamma Fc region receptor IIIB, neutrophil-specific antigen NA

Isotype Controls