This antibody recognises a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells. CD147 is also present on some endothelia. The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7. Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use10ul of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
T blasts from a mixed lymphocyte reaction.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-47
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Rat CD147 antibody, clone OX-47 recognizes a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells (Paterson et al. 1987). CD147 is also present on some endothelia (Fossum et al. 1991). The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue (Fossum et al. 1991).CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Foda et al. 2001).