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Anti-CD146 Antibody (A88042)

Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.com Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.com Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.com Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.com Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.com Immunofluorescence - Anti-CD146 Antibody (A88042) - Antibodies.com Immunofluorescence - Anti-CD146 Antibody (A88042) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD146 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD146 Antibody
Description
Rabbit polyclonal antibody to CD146.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-559 of human CD146/MCAM (NP_006491.2).
Sequence
VPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
A32 antigen, CD 146, CD146 antigen, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Gicerin, Mcam, Melanoma adhesion molecule, Melanoma associated antigen A32, Melanoma associated antigen MUC18, Melanoma associated glycoprotein MUC18, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MelCAM, MUC 18, MUC18, MUC18_HUMAN, S endo 1, S endo 1 endothelial associated antigen, S-endo 1 endothelial-associated antigen
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD146 Antibody (A88042)
Western blot analysis of extracts of various cell lines, using Anti-CD146 Antibody (A88042) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-CD146 Antibody (A88042) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-CD146 Antibody (A88042)
Western blot analysis of extracts of various cell lines, using Anti-CD146 Antibody (A88042) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD146 Antibody (A88042)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CD146 Antibody (A88042) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD146 Antibody (A88042) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD146 Antibody (A88042)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-CD146 Antibody (A88042) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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