Anti-CD14 Antibody [MEM-18] (FITC) (A281111)

$145

Mouse monoclonal [MEM-18] antibody to CD14 (FITC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD14 Antibody [MEM-18] (FITC)
Description
Mouse monoclonal [MEM-18] antibody to CD14 (FITC).
Specificity
This antibody recognises human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognises the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagentMouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes and has been used successfully for the detection of soluble CD14 in saliva samples.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl or 100µl whole blood
Reactivity
Human
Immunogen
Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-18
Isotype
IgG1
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagentMouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prager et al. 2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandi et al. 2007).
Synonyms
CD 14, CD14 antigen, CD14 molecule, CD14_HUMAN, CD_antigen=CD14, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell specific leucine rich glycoprotein, Myeloid cell-specific leucine-rich glycoprotein
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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