Anti-CD133 Antibody (A12549)

Name: Anti-CD133 Antibody
Brand: Antibodies.com
SKU: A12549
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CD133 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CD133 Antibody

Description

Rabbit polyclonal antibody to CD133.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 750-850 of human CD133 (NP_006008.1).

Sequence

FEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AC133, Antigen AC133, CORD12, Hematopoietic stem cell antigen, hProminin, MCDR2, MSTP061, OTTHUMP00000217744, OTTHUMP00000217745, OTTHUMP00000217746, PROM1, PROM1_HUMAN, Prominin I, Prominin like 1, Prominin like protein 1 precursor, Prominin mouse like 1, Prominin-1, Prominin-like protein 1, Prominin1, PROML1, RP41, STGD4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CD133 Antibody (A12549)

Western blot analysis of extracts of HCT116 cells, using Anti-CD133 Antibody (A12549) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-CD133 Antibody (A12549)

Western blot analysis of extracts of various cell lines, using Anti-CD133 Antibody (A12549) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunofluorescence - Anti-CD133 Antibody (A12549)

Immunofluorescence analysis of NIH/3T3 cells using Anti-CD133 Antibody (A12549) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).