This antibody recognises the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150 - 170 kDa.CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion. Mouse anti Horse CD13 antibody, clone CVS19 may be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.In addition to clone CVS19, other CVS clones recognising equine MHC and cell surface antigens are available.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reactivity

Horse

Immunogen

Equine leucocytes.

Host

Mouse

Clonality

Monoclonal

Clone ID

CVS19

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Horse CD13 antibody, clone CVS19 recognizes the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150 - 170 kDa.CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion (Saiki et al. 1993).Mouse anti Horse CD13 antibody, clone CVS19 may be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.In addition to clone CVS19, other CVS clones recognising equine MHC and cell surface antigens are available.

Synonyms

Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13 antigen, gp150, hAPN, LAP 1, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN

Isotype Controls