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Anti-CD13 Antibody (A14890)

Western Blot - Anti-CD13 Antibody (A14890) - Antibodies.com Western Blot - Anti-CD13 Antibody (A14890) - Antibodies.com Immunohistochemistry - Anti-CD13 Antibody (A14890) - Antibodies.com Immunohistochemistry - Anti-CD13 Antibody (A14890) - Antibodies.com Immunohistochemistry - Anti-CD13 Antibody (A14890) - Antibodies.com Immunofluorescence - Anti-CD13 Antibody (A14890) - Antibodies.com Immunofluorescence - Anti-CD13 Antibody (A14890) - Antibodies.com +1
$335
Product Datasheet

Rabbit polyclonal antibody to CD13 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD13 Antibody
Description
Rabbit polyclonal antibody to CD13.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 668-967 of human CD13/CD13/ANPEP (NP_001141.2).
Sequence
LASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
150 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13 antigen, gp150, hAPN, LAP 1, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD13 Antibody (A14890) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD13 Antibody (A14890)
Western blot analysis of extracts of various cell lines, using Anti-CD13 Antibody (A14890) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-CD13 Antibody (A14890) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-CD13 Antibody (A14890)
Western blot analysis of extracts of OVCAR3 cells, using Anti-CD13 Antibody (A14890) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-CD13 Antibody (A14890) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD13 Antibody (A14890)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD13 Antibody (A14890) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD13 Antibody (A14890) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD13 Antibody (A14890)
Immunohistochemistry analysis of paraffin-embedded mouse intestin using Anti-CD13 Antibody (A14890) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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