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Anti-CD127 Antibody [ARC0672] (A308134)

Western Blot - Anti-CD127 Antibody [ARC0672] (A308134) - Antibodies.com Immunofluorescence - Anti-CD127 Antibody [ARC0672] (A308134) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0672] antibody to CD127 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-CD127 Antibody [ARC0672]
Description
Rabbit monoclonal [ARC0672] antibody to CD127.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CD127/IL7R (P16871).
Sequence
PESFLDCQIHRVDDIQARDEVEGFLQDTFPQQLEESEKQRLGGDVQSPNCPSEDVVITPESFGRDSSLTCLAGNVSACDAPILSSSRSLDCRESGKNGPHV
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0672
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
72 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD 127, CD127 antigen, CDw127, IL 7R, IL 7R alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, IL7R alpha, IL7RA, IL7Ralpha, IL7RA_HUMAN, ILRA, Interleukin 7 receptor, Interleukin 7 receptor alpha chain, Interleukin 7 receptor isoform H5 6, Interleukin-7 receptor subunit alpha
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD127 Antibody [ARC0672] (A308134) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD127 Antibody [ARC0672] (A308134)
Western blot analysis of extracts of various cell lines, using Anti-CD127 Antibody [ARC0672] (A308134) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-CD127 Antibody [ARC0672] (A308134) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-CD127 Antibody [ARC0672] (A308134)
Immunofluorescence analysis of K562 cells using Anti-CD127 Antibody [ARC0672] (A308134) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CD127 Antibody [ARC0672] (A308134)? Please let us know so that we can list the citation on this page.

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