Mouse monoclonal [MEM-229] antibody to CD105 (PerCP-Cyanine 5.5).
Specificity
This antibody recognizes an extracellular epitope of CD105, a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow, and erythroid precursors in fetal and adult bone marrow. It is also present on syncytiotrophoblast on placenta throughout pregnancy.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Porcine, Human
Cross Reactivity
This antibody does not cross react with Dog or Horse.
Immunogen
Recombinant Vaccinia virus containing the human CD105 (L isoform) cDNA.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-229
Isotype
IgG2a
Conjugate
PerCP-Cyanine 5.5
Excitation: 482nm, Emission: 695nm
Purification
This antibody is conjugated with tandem dye PerCP-Cyanine 5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
90 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of CD105 on HUVEC cells with Anti-CD105 Antibody [MEM-229] (PerCP-Cyanine 5.5).
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