Recombinant Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533)

Name: Anti-CD10 Antibody [DM176] - BSA and Azide free
Brand: Antibodies.com
SKU: A318533
Price: 665.0 USD
Availability: InStock

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$665

Recombinant rabbit monoclonal [DM176] antibody to CD10 for ELISA and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Recombinant Antibody Mesenchymal Stem Cell Marker

Specifications

Name

Anti-CD10 Antibody [DM176] - BSA and Azide free

Description

Recombinant rabbit monoclonal [DM176] antibody to CD10.

Applications

ELISA, Flow Cytometry

Dilutions

ELISA: 1:5,000-10,000, Flow Cytometry: 1:100

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Clone ID

DM176

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity chromatography.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with distilled sterile water.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

General Notes

Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Synonyms

Atriopeptidase, CALLA, CD10 antigen, Common acute lymphocytic leukemia antigen, DKFZp686O16152, EC 3.4.24.11, Enkephalinase, EPN, Membrane metallo endopeptidase, Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase), Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10), Membrane metallo endopeptidase variant 1, Membrane metallo endopeptidase variant 2, Membrane metalloendopeptidase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10, Membrane metalloendopeptidase variant 1, Membrane metalloendopeptidase variant 2, MGC126681, MGC126707, MME, NEP, Neprilysin, neprilysin-390, neprilysin-411, NEP_HUMAN, Neutral endopeptidase, Neutral endopeptidase 24.11, Neutral endopeptidase, membrane-associated, SFE, Skin fibroblast elastase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Flow Cytometry - Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533)

CD10 protein is expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533) on Expi293 cells transfected with human CD10 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

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Figure 2: Flow Cytometry - Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533)

Flow cytometry analysis of antigen binding of Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533). (A) Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533) does not bind to 293T cells that do not express CD10. (B) A clear peak shift of Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533) was seen compared to the control when incubated with CD10-expressing Raji cells, indicating strong binding of Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533) to CD10. Antibodies were incubated at 5 µg/ml.

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Figure 3: Flow Cytometry - Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533)

Flow cytometry analysis of antigen binding of Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533). (A) Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533) does not bind to 293T cells that do not express CD10. (B) A clear peak shift of Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533) was seen compared to the control when incubated with CD10-expressing HT55 cells, indicating strong binding of Anti-CD10 Antibody [DM176] - BSA and Azide free (A318533) to CD10. Antibodies were incubated at 5 µg/ml.

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