This antibody recognises mouse C-C chemokine receptor type 7, also known as CD197, CCR7, Epstein-Barr virus-induced G-protein coupled receptor 1 or MIP-3 beta receptor. CD197 is a 378 amino acid ~43 kDa multi pass type III transmembrane glycoprotein and member of the G-protein coupled receptor 1 family (UniProt: P74447). CD197 is expressed by various lymphoid tissues, activated B and T cells, naive T cells, NK cells and dendritic cells (DCs), which acts a high-affinity receptor for the chemokines MIP-3beta (CCL19) and CCL21.Ligand binding of CD197 plays a vital role in the homing of naive T cells and antigen-loaded DCs into secondary organs. Expression of CD197 is strongly up-regulated in B cells infected with Epstein-Barr virus (EBV) and T cells infected with herpesvirus 6 or 7. Goat anti Mouse CD197 antibody can be used to distinguish between central memory and effector memory T cells and is suitable for use in immunocytochemistry.

Applications

WB, IHC-P, ELISA, Flow Cytometry, Functional Assays

Dilutions

ELISA: 1:100,000, Flow Cytometry: 1:10, Functional Assays: 8ug, IHC-P: 1:250, WB: 1:1,000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Reactivity

Mouse, Human

Immunogen

Synthetic peptide sequence DPGKPRKNVLVVALLVIFQVC corresponding to amino acids 2-22 of mouse CD197.

Sequence

DPGKPRKNVLVVALLVIFQVC

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.1% Sodium Azide.

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Goat anti Mouse CD197 antibody recognizes mouse C-C chemokine receptor type 7, also known as CD197, CCR7, Epstein-Barr virus-induced G-protein coupled receptor 1 or MIP-3 beta receptor. CD197 is a 378 amino acid ~43 kDa multi pass type III transmembrane glycoprotein and member of the G-protein coupled receptor 1 family (UniProt: P74447). CD197 is expressed by various lymphoid tissues, activated B and T cells, naive T cells, NK cells and dendritic cells (DCs), which acts a high-affinity receptor for the chemokines MIP-3beta (CCL19) and CCL21.Ligand binding of CD197 plays a vital role in the homing of naive T cells and antigen-loaded DCs into secondary organs. Expression of CD197 is strongly up-regulated in B cells infected with Epstein-Barr virus (EBV) and T cells infected with herpesvirus 6 or 7. Goat anti Mouse CD197 antibody can be used to distinguish between central memory and effector memory T cells and is suitable for use in immunocytochemistry.

Synonyms

BLR 2, BLR2, C C chemokine receptor type 7, C C CKR 7, C-C chemokine receptor type 7, C-C CKR-7, CC chemokine receptor 7, CC chemokine receptor type 7, CC CKR 7, CC-CKR-7, CCCKR7, CCR 7, CCR-7, CCR7_HUMAN, CD 197, CD197, CD197 antigen, CDw197, Chemokine (C C motif) receptor 7, Chemokine (C C) receptor 7, Chemokine C C motif receptor 7, Chemokine C C receptor 7, Chemokine receptor 7, Chemokine receptor 7-like protein, CMKBR7, EBI 1, EBI1, Ebi1h, EBV Induced G Protein Coupled Receptor 1, EBV-induced G-protein coupled receptor 1, Epstein Barr virus induced G protein coupled receptor, Epstein Barr virus induced gene 1, Epstein-Barr virus-induced G-protein coupled receptor 1, EVI 1, EVI1, Lymphocyte Specific G Protein Coupled Peptide Receptor, MGC108519, MIP 3 beta receptor, MIP-3 beta receptor, MIP3 Beta Receptor

Isotype Controls