Figure 1: Western Blot - Anti-CCNA2 Antibody (A15815)
Western blot analysis of extracts of various cell lines, using Anti-CCNA2 Antibody (A7632) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using Anti-CCNA2 Antibody (A7632) at dilution of 1:100 (40x lens).