Anti-MCP1 Antibody (A12099)

Name: Anti-MCP1 Antibody
Brand: Antibodies.com
SKU: A12099
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to MCP1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MCP1 Antibody

Description

Rabbit polyclonal antibody to MCP1.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human CCL2/MCP-1 (NP_002973.1).

Sequence

QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

13 - 15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C-C motif chemokine 2, CCL2, CCL2_HUMAN, Chemokine (C C motif) ligand 2, GDCF 2, GDCF-2, GDCF2, HC11, HSMCR30, JE, MCAF, MCP 1, MCP-1, MGC9434, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je), Small inducible cytokine subfamily A (Cys Cys), member 2, Small-inducible cytokine A2, SMC CF, SMC-CF, SMCCF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-MCP1 Antibody (A12099)

Western blot analysis of A-549, using Anti-MCP1 Antibody (A12099) at 1:2,000 dilution. A-549 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 2: Western Blot - Anti-MCP1 Antibody (A12099)

Western blot analysis of various lysates, using Anti-MCP1 Antibody (A12099) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-MCP1 Antibody (A12099)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-MCP1 Antibody (A12099) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MCP1 Antibody (A12099)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-MCP1 Antibody (A12099) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.