Anti-CBR1 Antibody (A83856)

Name: Anti-CBR1 Antibody
Brand: Antibodies.com
SKU: A83856
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to CBR1 for ELISA, WB, IHC and IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CBR1 Antibody

Description

Goat polyclonal antibody to CBR1.

Applications

ELISA, WB, IHC, IF

Dilutions

ELISA: 1:4,000, WB: 0.03-0.1µg/ml, IHC: 5µg/ml, IF: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human CBR1.

Sequence

C-HGQFVSEKRVEQW

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

30-35 kDa

Predicted MW

30.4kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

15 hydroxyprostaglandin dehydrogenase [NADP+], 15-hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl Reductase 1, Carbonyl reductase [NADPH] 1, CBR 1, CBR1_HUMAN, CRN, NADPH dependent carbonyl reductase 1, NADPH-dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin 9-ketoreductase, Prostaglandin E(2) 9 reductase, Prostaglandin-E(2) 9-reductase, SDR21C1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-CBR1 Antibody (A83856)

CBR1 expression in Human Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CBR1 Antibody (A83856) at 0.03µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-CBR1 Antibody (A83856)

CBR1 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CBR1 Antibody (A83856) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunohistochemistry - Anti-CBR1 Antibody (A83856)

CBR1 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-CBR1 Antibody (A83856) at 5µg/ml and revealed with alkaline phosphatase (AP).

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