Western blot analysis of 293T, using Anti-CBL Antibody (A87609) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western blot analysis of Mouse thymus, using Anti-CBL Antibody (A87609) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human prostate using Anti-CBL Antibody (A87609) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-CBL Antibody (A87609) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of K-562 cells using Anti-CBL Antibody (A87609) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-CBL Antibody (A87609) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-CBL Antibody (A87609) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 600µg extracts of mouse thymus cells using 3µg of Anti-CBL Antibody (A87609). This Western blot was performed on the immunoprecipitate using Anti-CBL Antibody (A87609) at a dilution of 1:1000.
