Anti-Cathepsin B Aptamer [Apt08rc] (Biotin) (A321160)

Anti-Cathepsin B Aptamer [Apt08rc] (Biotin)

Synthetic DNA aptamer to Cathepsin B [Apt08rc] (Biotin).

Apt08rc

Biotin

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

Binding Buffer: Phosphate Buffered Saline, pH 7.4, with 1mM Calcium Chloride·2 H2O and 0.5mM Magnesium Chloride·6 H2O. Washing Buffer: Binding Buffer, pH 8, Tween 20, and 0.05% (v/v). Binding Temperature: 20°C.

5'-dGpdGpdGpdCpdApdGpdGpdCpdApdApdCpdCpdTpdCpdCpdCpdApdCpdTpdApdTpdTpdGpdApdApdGpdApdCpdApdCpdTpdApdCpdCpdApdCpdCpdApdCpdCpdApdCpdTpdGpdCpdApdCpdApdCpdAp-3'

50 nt

Affinity (Kd) = 10.1-10.7 nM

HPLC

Lyophilized

Reconstitution dependent.

Lyophilized from nuclease-free water (no residual salts).

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.