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Rabbit Anti-Sheep IgG H&L Antibody (HRP) (A17336)

Western Blot - Rabbit Anti-Sheep IgG H&L Antibody (HRP) (A17336) - Antibodies.com
$100
Product Datasheet

Rabbit Anti-Sheep IgG H&L Antibody (HRP) for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Rabbit Anti-Sheep IgG H&L Antibody (HRP)
Description
Rabbit Anti-Sheep IgG H&L Antibody (HRP).
Host Species
Rabbit
Target Species
Sheep
Applications
WB, IHC
Recommended Dilutions
WB: 1:5,000-1:10,000, IHC: 1:500-1:5,000
Immunogen
Sheep IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate

HRP

Product Form
Liquid
Purification
Affinity purification.
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.75% BSA.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Rabbit Anti-Sheep IgG H&L Antibody (HRP) (A17336) - Antibodies.comEnlarge Image

Western Blot - Rabbit Anti-Sheep IgG H&L Antibody (HRP) (A17336)

Western blot analysis of extracts of A-431 cells, using Rabbit Anti-Sheep IgG H&L Antibody (HRP) (A17336) at 1:1,000 dilution or EGFR antibody (A11351). A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
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