ELISA of mouse immunoglobulins shows Anti-Mouse IgG3 Antibody [RM218] reacts to both mouse IgG3? and IgG3?. There is no cross-reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG3 Antibody [RM218] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of Anti-Mouse IgG3 Antibody [RM218] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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