Western Blot - Anti-Mouse IgG1 Antibody [RM106] (Biotin) (A121284)
Western blot of non-reduced (-) and reduced (+) mouse IgG1 (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG1 Antibody [RM106] (Biotin). This antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced ?1 form (~50 kDa).
ELISA of mouse immunoglobulins shows Anti-Mouse IgG1 Antibody [RM106] reacts to the Fc region of mouse IgG1. There is no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG1 Antibody [RM106] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of Anti-Mouse IgG1 Antibody [RM106] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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