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Recombinant Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin) (A121254)

Western Blot - Anti-Mouse Ig Lambda Light Chain Antibody (Biotin) [RM110] (A121254) - Antibodies.com ELISA - Anti-Mouse Ig Lambda Light Chain Antibody (Biotin) [RM110] (A121254) - Antibodies.com ELISA - Anti-Mouse Ig Lambda Light Chain Antibody (Biotin) [RM110] (A121311) - Antibodies.com
$495

Recombinant rabbit monoclonal [RM110] antibody to Mouse Mouse Ig Lambda Light Chain (Biotin) for ELISA and WB (non-reduced).

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin)
Description
Recombinant rabbit monoclonal [RM110] antibody to Mouse Mouse Ig Lambda Light Chain (Biotin).
Host Species
Rabbit
Target Species
Mouse
Specificity
This antibody recognizes the lamda light chain of mouse immunoglobulins.
Applications
ELISA, WB (non-reduced)
Recommended Dilutions
ELISA: 0.005-2 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.1-0.5 µg/ml
Immunogen
Mouse IgM Lambda.
Antigen Species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein A chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Mouse Ig Lambda Light Chain Antibody (Biotin) [RM110] (A121254) - Antibodies.comEnlarge Image

Western Blot - Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin) (A121254)

Western blot of non-reduced (-) and reduced (+) mouse IgG1? and IgG1? (20ng/lane), using 0.2µg/ml of Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin). This antibody reacts to non-reduced IgG1?.
ELISA - Anti-Mouse Ig Lambda Light Chain Antibody (Biotin) [RM110] (A121254) - Antibodies.comEnlarge Image

Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin) (A121254)

ELISA of mouse immunoglobulins shows Anti-Mouse Ig Lambda Light Chain Antibody [RM110] reacts to the lambda light chain of mouse immunoglobulins. There is no cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse Ig Lambda Light Chain Antibody [RM110] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Mouse Ig Lambda Light Chain Antibody (Biotin) [RM110] (A121311) - Antibodies.comEnlarge Image

Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin) (A121254)

A titer ELISA of mouse IgG1?. The plate was coated with different amounts of mouse IgG1?. A serial dilution of Anti-Mouse Ig Lambda Light Chain Antibody [RM110] was used as the primary antibody.An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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