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Goat Anti-Mouse IgG H&L Antibody (FITC), Cross-Absorbed (A282461)

$340

Goat anti-mouse IgG H&L (FITC) secondary antibody for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Goat Anti-Mouse IgG H&L Antibody (FITC), Cross-Absorbed
Description
Goat anti-mouse IgG H&L (FITC) secondary antibody.
Host Species
Goat
Target Species
Mouse
Specificity
This antibody recognises mouse IgG and light chains common to other mouse immunoglobulin classes.Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFα and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry.
Applications
Flow Cytometry
Recommended Dilutions
Flow Cytometry: 1:50 - 1:200, Use 50 µl of the suggested working dilution to label 1x106 cells in 100µl
Immunogen
Whole mouse IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Product Form
Liquid
Concentration
500 µg/ml
Purification
Affinity chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 0.2% BSA and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Goat anti Mouse IgG antibody recognizes mouse IgG and light chains common to other mouse immunoglobulin classes.Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFa and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry (Redondo et al. 2013).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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