This antibody recognises both the heavy and light chains of mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues. We recommend diluting this product in buffer containing 10% normal rat serum to remove any remaining cross-reactivity.
Applications
Flow Cytometry
Recommended Dilutions
Flow Cytometry: 1:5, Use 50ul of the suggested working dilution to label 106 cells in 100µl.
Immunogen
Mouse IgG, whole molecule.
Clonality
Polyclonal
Isotype
IgG
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
RPE conjugated Goat anti Mouse IfF antibody recognizes both the heavy and light chains of mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues. We recommend diluting this product in buffer containing 10% normal rat serum to remove any remaining cross-reactivity.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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