This antibody recognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues. We recommend diluting Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
Applications
Flow Cytometry
Recommended Dilutions
Flow Cytometry: 1:5 - 1:10, Use 50ul of the suggested working dilution to label 106 cells in 100µl.
Immunogen
Whole rat IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody recognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues. We recommend diluting Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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