Donkey F(ab')2 Anti-Rabbit IgG H&L Antibody (AP), Cross-Adsorbed (A294929)

Donkey F(ab')2 anti-rabbit IgG H&L (AP) secondary antibody for WB, IHC, ICC and ELISA.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.
Name
Donkey F(ab')2 Anti-Rabbit IgG H&L Antibody (AP), Cross-Adsorbed
Description
Donkey F(ab')2 anti-rabbit IgG H&L (AP) secondary antibody.
Host Species
Donkey
Target Species
Rabbit
Applications
WB, IHC, ICC, ELISA
Recommended Dilutions
ELISA: 1:1,000-1:20,000; ELISA (capture antibody): 1:50-1:250, ICC: 1:200-1:2,000, IHC: 1:200-1:2,000, WB: 1:1,000-1:20,000
Clonality
Polyclonal
Isotype
IgG
Conjugate

AP

Product Form
Liquid
Concentration
500 µg/ml
Purity
Antigen affinity purification.
Purification
Antigen affinity purification.
Formulation
Supplied in 50 mM HEPES, pH 7.1, with 0.1 M Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 0.2% BSA, and 0.09% Sodium Azide.
Storage
Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze!
General Notes
Antiserum was cross-adsorbed using bovine, chicken, goat, human, mouse, rat, and sheep immunosorbents to remove cross reactive antibodies. This antibody was then isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion, and Fc fragments and whole IgG molecules were removed. F(ab')2 fragments were then conjugated to alkaline phosphatase (AP).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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