Synthetic Nanodisc Human Transmembrane 4 L6 Family Member 1 Protein
Description
Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human Transmembrane 4 L6 Family Member 1 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human Transmembrane 4 L6 Family Member 1 protein has a MW of 21.6 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synthetic Nanodisc Human Transmembrane 4 L6 Family Member 1 Protein (A318476)
ELISA plates were pre-coated with Synthetic Nanodisc Human Transmembrane 4 L6 Family Member 1 Protein (A318476) (0.2 µg/well). Serial diluted Anti-Transmembrane 4 L6 Family Member 1 Humanized Antibody [AGX101 Biosimilar] - Azide free (A318815) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-TM4SF1 Monoclonal Antibody binding with TM4SF1-Nanodisc is 15.97 µg/ml.
Synthetic Nanodisc Human Transmembrane 4 L6 Family Member 1 Protein (A318476)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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