Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human STEAP2 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human STEAP2 protein has a MW of 56.1 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
Metalloreductase STEAP2, PCANAP1, Prostate cancer-associated protein 1, Protein up-regulated in metastatic prostate cancer, PUMPCn, Six-transmembrane epithelial antigen of prostate 2, SixTransMembrane protein of prostate 1, STAMP1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA plates were pre-coated with Synthetic Nanodisc Human STEAP2 Protein (A318457) (0.2 µg/well). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-Flag Tag Antibody binding with STEAP2-Nanodisc is 9.198 µg/ml.
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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