Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR TAS1R2 protein to be purified and analysed in vitro.
Applications
SDS-PAGE, ELISA
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human GPCR TAS1R2 protein has a MW of 95.2 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
G-protein coupled receptor 71, GPR71, Sweet taste receptor T1R2, T1R2, TAS1R2, Taste receptor type 1 member 2, TR2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Synthetic Nanodisc Human GPCR TAS1R2 Protein (A325182)
ELISA plates were pre-coated with Synthetic Nanodisc Human GPCR TAS1R2 Protein (A325182) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human GPCR TAS1R2 Protein (A325182) is 4.703ng/ml.
Synthetic Nanodisc Human GPCR TAS1R2 Protein (A325182)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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